SequenceVariation ina-Methylacyl-CoARacemase andRiskof Early-Onset and Familial ProstateCancer

BACKGROUND. Expression of the a-methylacyl-CoA racemase (AMACR) gene has been established as a sensitive and specific biomarker for the diagnosis of prostate cancer. An initial study has also suggested that the risk of familial (but not sporadic) prostate cancer may be associated with germline variation in the AMACR gene. METHODS. In a study of brothers discordant for the diagnosis of prostate cancer (including 449 affected and 394 unaffectedmen) from 332 familial and early-onset prostate cancer families, we used conditional logistic regression and family-based association tests to investigate the association between prostate cancer and five single nucleotide polymorphisms (SNPs) tagging common haplotype variation within the coding and regulatory regions of AMACR. RESULTS. The strongest evidence for prostate cancer associationwas for SNP rs3195676, with an estimated odds ratio of 0.58 (95% confidence interval1⁄4 0.38–0.90; P1⁄4 0.01 for a recessive model). This non-synonymous SNP (nsSNP) results in a methionine-to-valine substitution at codon 9 (M9V) in exon 2 of the AMACR gene. Three additional nsSNPs showed suggestive evidence for prostate cancer association (P 0.10). CONCLUSIONS. Our results confirm an initial report of association between the AMACR gene and the risk of familial prostate cancer. These findings emphasize the value of studying early-onset and familial prostate cancer when attempting to identify genetic variation associated with prostate cancer. Prostate 67: 1507–1513, 2007. # 2007 Wiley-Liss, Inc.